Flow Cytometry Basics:
Flow cytometry is a method of looking cells or other suitable particles using fluorescence and qualities of light scattering. There are several excellent introductions to flow cytometry and cell sorting readily available on the internet. Here are just a few of the most useful sites.
- Introduction to Flow Cytometry — From ABD Serotec. A concise and informative overview of flow cytometry and cell sorting principles and practices. Basic information everyone should be familiar with before embarking upon a flow cytometry experiment.
- Compensation: An informal perspective — From Mario Roederer. One of the most respected leaders in the field of flow cytometry provides a detailed look at fluorescence compensation and how it can make or break your multi-color assay results.
- Practical Flow Cytometry, 4th edition — by Howard Shapiro. Far from “just the basics”, this is THE textbook for all things flow cytometry. The fourth edition is kindly available online for free and can also easily be accessed from your mobile device.
- Fluorescence Spectrum Viewer – From BD. This web-based interactive tool can assist you in your search for the right combination of colors for your experiment and show the amount of spillover between fluorochromes.
- Table of Fluorochromes – From the Salk Institute. Basic but useful information about a wide variety of fluorescent dyes and proteins.