Cytometry and Sorting Policies

New Users:

Before setting up the first sample run, new users must meet with the core technical manager to discuss the experiment. This will ensure that the user’s needs can be met and that the core’s standard operating procedures are followed.

If your research is done according to an MU Institutional Biosafety Committee (IBC) protocol, an amendment must be submitted to add the LIDR Immunology Core to your protocol. If we have not worked with your organism before, the IC will have to add it to our own protocol as well. Please allow at least a couple of weeks for this process.

All samples must be filtered with a 40 micron or smaller cell strainer immediately prior to bringing the samples to the IC, even if filtering is an earlier step in your cell preparation protocol. Filters can be purchased from Fisher Scientific as catalog number 22-363-547.

If your samples are fixed BSL-3 agents, you must validate your fixation protocol and submit it to the biosafety officer.

Existing Users:

To make an appointment for analysis, or sorting, contact the core technical manager. If any antibodies, fluorescent conjugants, cell-types or infection states have changed since the last experiment, make sure to let her know in your email. Even if all aspects of your experiment are the same, you must fill out and bring a cytometry information sheet and a sorting information sheet (if applicable) to each appointment.

Reminder: All samples must be filtered with a 40 micron or smaller cell strainer immediately prior to bringing the samples to the IC, even if filtering is an earlier step in your cell preparation protocol.

Fluorescence Microscopy Policies

Contact us for more information.

Fees

(rev. 06/02/2012)

Flow cytometric analysis (operator-assisted only):

• University of Missouri research – $85.00 per hour
• Other academic or industry users – contact us

Cell sorting (operator-assisted only):

• University of Missouri research – $150.00 plus $100.00 per hour
• Other academic or industry users – contact us

Fluorescence Microscopy:

• There are no fees for use of the microscope at this time.